or placebo o relative to sham instrumented control hearts at top left panel, top right and min bottom left panel mean lvdp of hearts from to min of reperfusion are shown in the bottom right panel tetrazolium chloride stained heart slices showed that hearts treated with csil at , and testosterone monitoring hypogonadism min had similar injury as that of the sham operated hearts � , � and � , and � respectively p � ns the infarct size of hearts testosterone monitoring hypogonadism treated with csil even at min of global ischemia was � of the testosterone monitoring hypogonadism ventricles this was significantly smaller than its corresponding control p , whereas hearts testosterone monitoring hypogonadism treated with control iggl at , and min of global ischemia were � , � and � respectively fig , left panel the corresponding nitroblue tetrazolium chloride stained heart testosterone monitoring hypogonadism slices are shown in fig , right panel another parameter of myocardial injury testosterone monitoring hypogonadism that was determined was mitochondrial size although mitochondrial swelling is a hallmark of testosterone monitoring hypogonadism ischemic injury, irreversible injury cannot be directly extrapolated from just observation of mitochondrial size nevertheless, in view of the myocardial functional and his tochemical evidences, mitochondrial size assessment from transmission electron micrographs add additional support for myocardial preservation testosterone monitoring hypogonadism in csil treated hearts, relative to iggl or placebo treated hearts figure shows the comparison of fig mean infarct sizes of rat hearts treated with csil testosterone monitoring hypogonadism or iggl or placebo at and min of global ischemia left panel testosterone monitoring hypogonadism the corresponding nitroblue tetrazolium chloride stained mid slices of rat hearts treated with csil or iggl at , and min of global ischemia minimal injury was seen testosterone monitoring hypogonadism in and min csil treated hearts, but injury was evident in the min testosterone monitoring hypogonadism csil treated heart slice injury is evident in all heart slices treated testosterone monitoring hypogonadism with iggl right panel, bottom two rows �i i normal cut csil csil testosterone monitoring hypogonadism csejo igsl tg& iflsi placebo v fig mean mitochondrial size of normal, csil, testosterone monitoring hypogonadism iggl or placebo treated hearts treatment was as indicated in the text mitochondrial size of normal hearts, csil treatment at and min of global ischemia, as well as iggl treated hearts at , and min of global ischemia and testosterone monitoring hypogonadism with placebo no difference in mitochondrial size was observed between normal myocardium � mean number of pixels � sem and myocardium treated at and min of global ischemia � , � , � and � respectively p = ns however, mitochondria of hearts treated with iggl at , and min of global ischemia or placebo � , � , � and � respectively were larger than mitochondria of csil testosterone monitoring hypogonadism treated hearts p these studies showed that myocardial viability preservation is not restricted to embryonic cardiocytes in cultures adult hearts are also amenable to structural testosterone monitoring hypogonadism and functional preservation, following cell membrane lesion sealing in a timedependent manner during ischemia this method of cell membrane lesion sealing has also been reported to preserve the integrity of vascular endothelium with antiactinimmunoliposomes a question that remains testosterone monitoring hypogonadism concerning the utility of csil is whether immunoliposomes can retain the protective functions testosterone monitoring hypogonadism in the presence of plasma proteins in vivo, since experiments have demonstrated that testosterone monitoring hypogonadism cells in culture and adult hearts perfused with nonprotein oxygenated buffer were prevented from undergoing myocardial necrosis, following cell membrane lesion sealing intervention with cytoskeletal testosterone monitoring hypogonadism antigen specific immunoliposomes to demonstrate that cell membrane lesion sealing also occur in testosterone monitoring hypogonadism vivo, rabbits with experimental myocardial infarction were used in this study, rabbits were injected with antimyosin csil, plain liposomes or saline at the time of left circumflex coronary artery occlusion by intracoronary infusion the occlusion was kept for testosterone monitoring hypogonadism min followed by hrs of reperfusion the hearts were excised, sliced into � slices parallel to the short axis and stained with nitroblue tetrazolium chloride the infarct was approximately to of the infarcts of the control plain liposome testosterone monitoring hypogonadism or saline treated rabbit hearts subsequently, comparison to iggliposome treated hearts with acute testosterone monitoring hypogonadism myocardial infarction demonstrated that the csil treatment resulted in significantly smaller infarct size, as was observed in comparison to plain liposome or saline treated hearts thus, cytoskeletalantigen specific immunoliposomes, consisting of antimyosin or antiactinimmunoliposomes, were demonstrated to be testosterone monitoring hypogonadism able to preserve cell viability and integrity its potential utility in the cardiovascular testosterone monitoring hypogonadism system would be enhanced once its efficacy following intravenous delivery has been demonstrated however, the study of asahi et al showed that intravenous delivery of the antiactin immunoliposomes enabled preservation of the integrity of the endothelial cells of the cerebral vessels csil as targeted gene or drug delivery due to the proposed mechanism of cell membrane lesion sealing, we also proposed that if testosterone monitoring hypogonadism drugs or gene constructs were to be included in the immunoliposomes such as csils, then these drugs or gene constructs should be delivered directly into the testosterone monitoring hypogonadism cytoplasm fig this route should bypass the endocytic route of drugs or gene construct delivery, thereby reducing destruction of the delivered cargo by the lysosomal testosterone monitoring hypogonadism enzymes, after formation of endolysosomes using silver grains as model fig transmission electron testosterone monitoring hypogonadism micrographs of embryonic cardiocyte treated with silver grains impregnated csil left and plain liposome impregnated with silver grains right � = jim drugs, we demonstrated testosterone monitoring hypogonadism that these drugs can be delivered directly into the cytoplasm of hypoxic cardiocytes testosterone monitoring hypogonadism treated with silver grains loaded csils figure left shows a transmission electron micrograph testosterone monitoring hypogonadism of a cardiocyte treated with silver grains impregnated csils silver grains in testosterone monitoring hypogonadism groups of concentration at about nm were observed however, in cells treated with silver grains impregnated plain liposomes, very few cells were viable of one such testosterone monitoring hypogonadism cell detected by transmission electron microscopy, the silver grains were observed in the extracellular space [fig right] fig diagrammatic representation of delivery of intraliposomally entrapped testosterone monitoring hypogonadism genetic construct or drugs directly into the cytoplasm of target cell nmuin ftivoih wiiit mi ��� when the silver grains were replaced with genetic constructs, pgl testosterone monitoring hypogonadism and psv gal vectors, hypoxic cardiocytes treated with csil impregnated with either vectors showed luciferase activity or bacterial jgalactosidase activity the successful transfection of the hypoxic cardiocytes with pgl, a vector for firefly luciferase enzyme � � fig testosterone monitoring hypogonadism relative light units of luciferase activity of cardiocytes treated with various preparations and controls in csils is shown in fig as relative light units rlus rlus were determined by the use of a luminometer as can be seen, testosterone monitoring hypogonadism only hypoxic cardiocytes treated with pglcsils showed increased rlus significantly above normal cells testosterone monitoring hypogonadism with treatment with no vectors similarly, normoxic cardiocytes treated with pglcsil, hypoxic cardiocytes and normoxic cardiocytes treated with plain liposomes, or with only vectors, showed no significant gene transcription and expression when hypoxic cardiocytes were treated with csil testosterone monitoring hypogonadism with entrapped psvgal vectors, almost all cells in the field of view under testosterone monitoring hypogonadism light microscopy exhibited bacterial �galactosidase enzyme activity, following reaction with xgal bromo testosterone monitoring hypogonadism chloroindolylbetadgalactopyranoside, nm mgcl,mm iqfetcn h, mm kfecn in phosphate buffered saline ph [fig a] when this mode of gene expression was compared with transfection of psvjgal vector with cationic liposomes, cationic liposome transfection according to the manufacturers protocol resulted in transfection of only a few cells per field of view testosterone monitoring hypogonadism [fig b], in this micrograph, two cells with intense ��galactosidase activity were observed testosterone monitoring hypogonadism quantitation of the number of cells in the field of view that was testosterone monitoring hypogonadism successfully trans fected with psvjgal vector in csil, cationic liposome, iggliposomes, plain testosterone monitoring hypogonadism liposome and vector alone are shown in fig c only csil and cationic testosterone monitoring hypogonadism transfection showed gene expression csiltransfection or csilfection was more than times more efficient testosterone monitoring hypogonadism in transfecting cells than cationic liposomes although the intensity of gene expression was low with csilfection, using the initial vector concentration of xg of vectors testosterone monitoring hypogonadism in mg lipids in ml, when the vector concentration was increased to � testosterone monitoring hypogonadism zg, also in mg lipids in ml, the intensity of gene transfection was testosterone monitoring hypogonadism increased [fig d], this study showed that approximately x prozac interactions w high blood pressure xg ��� � m t� !