when looking at data soma methodology obtained from studies with living mammalian cells, it appears reasonable soma methodology to assume that dequalinium molecules soma methodology could be pulled into the mitochondrial matrix in response to soma methodology the high mitochondrial membrane potential soma methodology as demonstrated in , which in turn might lead to the soma methodology destabilization of the manufacturer of zocor dqasomepdna complex however, the first detailed study, which demonstrated the selective dna release from dqaplexes, was performed soma methodology using membrane mimicking liposomes fig as a model for the soma methodology intracellular release of dna from soma methodology dqasomes, the capacity of anionic liposomes to displace the dna from its cationic carrier was studied the association of dna with the cationic carrier was soma methodology assessed by employing sybr green i the fluorescence signal of this dye is greatly enhanced when bound to dna nonbinding results soma methodology in loss of fluorescence it soma methodology can be clearly seen that in the vicinity of a charge ratio, dqasomes do not soma methodology release any dna in the presence of cytoplasmic membrane mimicking liposomes dqasomes s � � o � u a � � � cpm imm ��� anionic liposomes i � � time [sec] fig effect of soma methodology anionic liposomes on dna release from dqasomepdna complexes dna was preincubated with sybr until stabilization of the signal, followed by adding indicated by arrow the minimal amount of dqasomes necessary to decrease the signal to background level anionic liposomes were then injected arrow at an anionic to cationic charge ratio as shown the displacement of soma methodology dna from its carrier is indicated by the increase of the fluorescence signal cpm, cytoplasma membrane like liposomes imm, inner mitochondrial membrane like liposomes omm, outer mitochondrial membrane like liposomes cpm, not even at a fold soma methodology excess of anionic charge however, soma methodology with a similar charge excess of anionic liposomes to cationic dqasomes, and respectively, inner and soma methodology outer mitochondrial membrane mimicking liposomes imm and omm, respectively are able to displace up to of the dna from its soma methodology dqasomal carrier in agreement with these data, it was found that for the complete liberation of dna from dna dqasome complex, a fourfold excess of soma methodology dicetylphosphate and an eightfold excess of phosphatidylserine, respectively, are necessary soma methodology the finding that cpm liposomes, soma methodology at an anionic to cationic charge ratio of , displace up soma methodology to of the dna from lipofectin, which was used as a control, do not liberate any soma methodology dna from dqasomes even at a slight excess of anionic charge, leads to the conclusion that besides the charge ratio, other factors may play an important role in the mechanism of dna release from lipiddna complexes this conclusion is being further supported by xu and szokas observation that ionic water soluble molecules such as atp, soma methodology trna, dna, polyglutamic acid, spermidine and histone do not displace dna from the cationic lipiddna soma methodology complex, even at a fold charge excess � in their model for the postendocytotic release of dna from cationic carriers, soma methodology they assume the formation allegra-d 12 hour of a charge neutral ion pair soma methodology between cationic and anionic lipid, which ultimately results in the displacement of the dna from the cationic lipid and the release of dna into cytoplasm liposomedna liposome ?